Dr. J. Brooks Crozier


Department: Biology
Office: 401A Life Science



B.S., Roanoke College; M.S., Ph.D., Virginia Polytechnic Institute & State University. 1999

Research & Teaching Interests

Microbiology, Mycology (fungi), Phycology (algae), INQ 250(about the microbiology and ecology of soil), INQ 300 (gives students an opportunity to learn about learning and solve a problem in the sciences).

Scholarly Activities

Microbial Source Tracking, including detection of virulence and host-specific DNA markers; performance criteria for such markers; and their application in target-oriented water resource management.

Sabbatical (Spring 2013) worked on several projects at Virginia Tech and spent April, May and part of June 2013 in the lab of Dr. Andreas Farnleitner, Vienna Austria.

Recent Publications

Hagedorn, C., A. Hassall Lawrence, J. B Crozier. 2014. Identifying Sources of Fecal Contamination in Shellfish Waters in Two Tidal Rivers. Journal of Shellfish Research. 33:207-216.

J. B. Crozier and M. Alvarez. 2011. Chapter 27. Training Future Scientists: Teaching Microbial Source Tracking (MST) to Undergraduates in Microbial Source Tracking: Methods, Applications, and Case Studies. Springer publishers.

J. W. Dickerson Jr., J. B. Crozier, C. Hagedorn, and A. Hassall. 2007. Assessment of the 16S-23S rDNA intergenic spacer region in Enterococcus spp. for Microbial Source Tracking. Journal of Environmental Quality. 36:1661-1669.

C. Hagedorn, J.B. Crozier, K.A. Mentz, A.M. Booth, A.K. Graves, N.J Nelson, and R.B. Reneau, Jr. 2003. Carbon Source utilization profiles as a method to identify sources of fecal pollution in water. Journal of Applied Microbiology 94 (5), 792-799.

J. B. Crozier. 2003. Development of a Bacterial Source Tracking Program at a Small Liberal Arts College. Environmental Detection News (4) 1-5.

J.B. Crozier, B. Clark, and H. Weber. 2002. Identifying Sources of Fecal Pollution in the Roanoke River, Roanoke County, Virginia. Virginia Journal of Science, 53:157-165.

Abstracts, poster presentations and other:

A.H Lawrence, J.B. Crozier, R. Farris, and C. Hagedorn. Fecal Source Identification in Impaired Waters in the National Capitol Region. 2013. Poster presented and Abstract. ASM General meeting, Denver, CO, May 2013.

Crozier, J. B. B. Badgely, C. Hagedorn. Water Quality and Microbial Community Analysis: Can Next Generation Sequencing be the Next DNA-Based Assay? Water Education Summit. September 2013. Chattanooga TN. Oral Presentation.

Mizelle, M. K. Mullins and J. B. Crozier. qPCR Detection of Human-specific Viral and Bacterial Fecal Markers in Comparison to Standard Fecal Indicator Bacteria. Poster and abstract. Virginia branch ASM conference, Norfolk VA, November 2012.

A. Hassall Lawrence, C. Hagedorn, J. Crozier, M. Saluta, R. Farris. 2012. Determining Sources of Fecal Pollution with qPCR-Bacteroidales in a Uniquely Biodiverse but Threatened Watershed. ASM 2012 General meeting. Poster presented and abstract.

Collins, R. and J. B. Crozier. 2012. The effects of fertilizer type on loblolly seedling growth:  Is one pellet better than two? Presented at North American Forest Ecology Workshop.

Scott, K, and J. Brooks Crozier.  2010. Duration of Detection of Human Bacteroidales Compared to Culturable Enterococcus in Water and sediment. Virginia Branch of the American Society for Microbiology, Lynchburg VA. Poster presented and abstract.

Hogan, L. and J. Brooks Crozier.  2010. Identification of Fungi on Treated Pine Roots by PCR Amplification and Sequencing of the Fungal ITS Region. Virginia Branch of the American Society for Microbiology, Lynchburg VA. Poster presented and abstract.

J. B. Crozier and K. Scott.  Bacteroides: DNA Extraction, Amplification, and Visualization.  June 2009.  Protocol written and presented day-long workshop for the Virginia Tech Hagedorn lab graduate student training workshop on PCR amplification of human-specific fecal markers. Workshop.

J. B. Crozier, J. Watkinson and K. Filer.  2010. Student Learning in an Undergraduate Non-Majors Soil Microbiology Course in a New Undergraduate General Education Curriculum. JMBE. 11:74-75. Poster presented and Abstract.

J. B. Crozier.  Microbial Source Tracking. 2006.  IN Pathogen Research Symposium: Pathways and Monitoring in Natural and Engineered Systems, Virginia Water Resources Research Center Special Report.

Hagedorn, C., Touchton, G., Hassall, A., and B. Crozier.  2006. Approaches to Source Classification of Fecal Bacterial Isolates from Water Samples to Improve Results from Library-Dependent Methods. ASM General Meeting, Orlando FL.  Poster presented and abstract.

J. B. Crozier, M. Salama, A. Hinlicky, C. Hagedorn, and A. Strand. Use of Escherichia coli fim Gene Polymorphisms as a Bacterial Source Tracking Tool. 2005, ASM General Meeting, Atlanta Georgia. Poster presented and abstract

J. B. Crozier, T. Simpson, M. Salama, F. Bosch. A Novel Method for the Source Tracking of Escherichia coli by Restriction Fragment Length Polymorphism (RFLP) Analysis of Amplified 16S-23S Internal Transcribed Spacer (ITS) Regions.  2003. ASM General Meeting, Washington, DC. Poster presented and abstract

William Dixon, Michael Bennett, James B. Crozier, Jon C. Cawley,   Rebecca Currie. Macroinvertebrates and diatoms in the Roanoke River.  2003. Southeastern/South Central National Meeting of the Geological Society of America, Memphis, TN. Poster presented and abstract

C. Hagedorn, B. Crozier, K. Hix, A. Booth, and A. Graves. Carbon Source Utilization Profiles as a Method to identify Sources      of Fecal Pollution in Water.  ASM, Salt Lake City, UT, May 2002. Poster presented and abstract

Invited lecture: Comparison of Bacterial Source Tracking Methods. presented to the National Environmental Health Association Annual Meeting, Atlanta, GA, June 28-July

3, 2001

J. B. Crozier. Fecal Source Tracking in the Roanoke River Using Antibiotic Resistance Patterns in the Enterococci. 2001. Southern Appalachian Man and the Biosphere (SAMAB) Annual Conference, Gatlinburg, TN.